PERBANDINGAN KADAR PROTEIN TOTAL MENGGUNAKAN SERUM, PLASMA EDTA DAN NATRIUM SITRAT DENGAN METODE BIURET

ABSTRACT

Total protein measurement is a useful test in a variety of disorders. A decrease in total protein concentration can be detected in impaired protein synthesis in the liver, protein loss due to impaired renal function, intestinal malabsorption, or nutritional deficiencies. Elevated protein levels occur in chronic inflammatory disorders, liver cirrhosis, and dehydration. Total protein examination can use venous blood made from plasma or serum. Making plasma from venous blood will have an osmotic effect due to the addition of anticoagulants which cause water to leave the cells and enter the plasma, thus thinning the plasma and reducing its concentration. The choice of specimen type during sample preparation can influence the results of examining total protein levels. In the photometric test according to the Biuret method, protein forms a purple-blue complex with copper ions in alkaline solutions. Color absorbance is directly proportional to concentration. Research has been carried out on the comparison of total protein examination results in serum samples, EDTA plasma samples and citrate plasma samples. The data obtained was an average total protein yield in serum of 7.30%, in EDTA plasma samples of 7.90%, and in citrate plasma of 6.76%. The data was processed statistically. The results obtained were processed using statistics in the form of normality tests, homogeneity tests, Kruskal Walis post hoc test, Mann-Whitney test and followed with the t-paired test. It was found that the total protein examination results in each data group were significantly different. This shows that specimen selection influences the examination results. It is recommended that for total protein examination, the sample chosen is fresh serum.

Key words: total protein levels, serum, plasma EDTA, plasma citrate, Biuret method