Dahaga, Anissa Maulidiansyah (2024) UJI EFEK ANTI BAKTERI EKSTRAK LIDAH BUAYA ( Aloe barbadensis miller ) TERHADAP PERTUMBUHAN Staphylococcus aureus. Diploma thesis, Sekolah Tinggi Analis Bakti Asih.
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Abstract
Acne or acne vulgaris is an inflammatory disorder of the pilosebaceous follicles. Acne affects 85% of the world's population aged 11-30 years. Bacteria that cause acne include Staphylococcus aureus. To treat acne used antibiotics. The use of antibiotics of natural compounds is one treatment that can be done against infection by bacteria. In Indonesia there are various types of plants that can be used as alternative medicinal ingredients, one of which is aloe vera (Aloe Barbadensis Miller). This study aims to determine the antibacterial effect of aloe vera extract (Aloe Barbadensis Miller) on the growth of Staphylococcus aureus. The research method used an experimental design with the Kirby Bauer diffusion method to see the inhibitory zone in isolates of Staphylococcus aureus bacteria and analyzed using the One-Way ANOVA statistical test with the SPSS program. The results of the study of natural aloe vera extract, packaged aloe vera extract and combined aloe vera extract produced an average inhibitory zone successively for 25% concentrations of 5 mm, 6 mm, and 5.6 mm, for 50% concentrations of 5.3 mm, 9 mm and 9 mm, for 75% concentrations of 8 mm, 10.3 mm and 10 mm. The higher the concentration of the extract, the larger the inhibitory zone formed. The results of packaged aloe vera extract with a concentration of 75% have the highest inhibitory zone value. The results of the Normality Test with the Shapiro-Wilk Test and the Variance Test obtained a significancy of >0.05 while the ANOVA Test obtained a significancy of >0.05 which means there is no significant difference between variations of aloe vera extract against the growth of Staphylococcus aureus bacteria. From the results of this study it was concluded that natural aloe vera skin extract, packaged aloe vera skin extract and combined aloe vera skin extract with concentrations of 25%, 50% and 75% have inhibitory power with lower sensitivity than erythromycin and there is no significant difference in antibacterial effects between natural aloe vera extract, packaged aloe vera extract and combination aloe vera extract.
Keywords : Aloe vera extract, antibacterial, Staphylococcus aureus
| Item Type: | Thesis (Diploma) |
|---|---|
| Additional Information: | ABSTRAK Jerawat atau acne vulgaris adalah gangguan inflamasi dari folikel pilosebasea. Jerawat menyerang 85% populasi dunia yang berusia 11-30 tahun. Bakteri penyebab jerawat diantaranya adalah Staphylococcus aureus. Untuk mengobati jerawat digunakan antibiotik. Penggunaan antibiotik senyawa bahan alam merupakan salah satu pengobatan yang dapat dilakukan terhadap infeksi oleh bakteri. Di Indonesia terdapat berbagai macam jenis tanaman yang dapat digunakan sebagai bahan alternatif obat salah satunya lidah buaya (Aloe Barbadensis Miller). Penelitian ini bertujuan untuk mengetahui efek antibakteri ekstrak lidah buaya (Aloe Barbadensis Miller) terhadap pertumbuhan Staphylococcus aureus. Metode penelitian menggunakan desain Eksperimental dengan metode difusi Kirby Bauer untuk melihat zona hambat pada isolat bakteri Staphylococcus aureus dan dianalisa menggunakan uji statistic One-Way ANOVA dengan program SPSS. Hasil penelitian ekstrak lidah buaya alami, ekstrak lidah buaya kemasan dan ekstrak lidah buaya kombinasi menghasilkan rata-rata zona hambat secara berturut-turut untuk konsentrasi 25% yaitu 5 mm, 6 mm, dan 5.6 mm, untuk konsentrasi 50% yaitu 5.3 mm, 9 mm dan 9 mm, untuk konsentrasi 75% yaitu 8 mm, 10.3 mm dan 10 mm. Semakin tinggi konsentrasi ekstrak maka zona hambat yang terbentuk semakin besar. Hasil ekstrak lidah buaya kemasan dengan konsentrasi 75% memiliki nilai zona hambat paling tinggi. Hasil Uji Normalitas dengan Uji Shapiro-Wilk dan Uji Varians didapatkan significancy >0.05 sedangkan pada Uji ANOVA didapatkan significancy >0.05 yang berarti tidak terdapat perbedaan bermakna antara variasi ekstrak lidah buaya terhadap pertumbuhan bakteri Staphylococcus aureus. Dari hasil penelitian ini didapat kesimpulan ekstrak kulit lidah buaya alami, ekstrak kulit lidah buaya kemasan dan ekstrak kulit lidah buaya kombinasi dengan konsentrasi 25%, 50% dan 75% memiliki daya hambat dengan sensitivitas lebih rendah dari Eritromisin serta tidak terdapat perbedaan bermakna efek antibakteri antara ekstrak lidah buaya alami, ekstrak lidah buaya kemasan dan ekstrak lidah buaya kombinasi. Kata kunci : Ekstrak lidah buaya, antibakteri, Staphylococcus aureus |
| Subjects: | Program Studi > Analis Kesehatan > Bakteriologi Program Studi > Analis Kesehatan |
| Divisions: | Analis Kesehatan > Bakteriologi |
| Depositing User: | Admin Repo |
| Date Deposited: | 04 Feb 2026 05:45 |
| Last Modified: | 04 Feb 2026 05:45 |
| URI: | https://repository.staba.ac.id/id/eprint/33 |
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