VARIASI PREPARASI SAMPEL TERHADAP PEMERIKSAAN HIV METODE IMUNOKROMATOGRAFI RAPID TEST

The problem of Human Immunodeficiency Virus (HIV) and Acquired Immunodeficiency Syndrome (AIDS) is still a serious problem and must be addressed immediately. Immunochromatography examination was chosen for rapid early detection of HIV infection which is effective and has a sensitivity of >99.5% and a specificity of >99.8%. Another advantage is that the results come out quickly in around 15-30 minutes, it is easy to do and does not require special tools. However, inconsistent sensitivity and specificity are often found to be due to a lack of knowledge about the characteristics of rapid test kits and the selection of the best sample type in the examination used as the sole test kit in the national HIV prevention and control program. The aim of this study was to compare the results of HIV testing with variations in sample preparation, namely samples that were not centrifuged (EDTA Blood/whole blood) and centrifuged (EDTA Plasma) in HIV testing using the rapid immunochromatography test method at the Pulo Armyn Public Health Center UPTD laboratory in February. 2024. The results of the HIV examination were carried out on 15 respondents with a total of 30 examination samples, through a data normality test using the Shapiro-Wilk test, in HIV examination with whole blood and EDTA plasma samples the sig value was <0.01 <0.05 and EDTA plasma samples If the sig value is 0.0 < 0.05, it is concluded that this test data is not normally distributed. Then proceed with the Anova Homogeneity test, the sig value was 0.015 > 0.05, which means Homogeneous, and finally the Mann Whitney U test obtained the Sig Asymp value (2 tailed) 0.015 > 0.05, so the conclusion is that there is an influence on the use of the sample or H0 is rejected and Ha accepted.

Keywords: Sample Variations, EDTA Blood, EDTA Plasma, Immunochromatography, HIV