PERBANDINGAN KONSENTRASI EKSTRAK DAUN JATI 1:2 1:3 DAN 1:4 PENGGANTI EOSIN PADA PEWARNAAN HEMATOXILIN EOSIN PADA JARINGAN GINJAL TIKUS PUTIH (Wistar)

Hematoxylin and eosin (HE) staining is a type of staining that is carried out by staining tissue. Dyes are divided into two types, namely synthetic dyes and natural dyes. Natural dyes are dyes made from natural ingredients. Natural dyes add color to tissue, making it visible under a microscope. Natural dyes do not contain toxic ingredients so they are not carcinogenic, and natural dye waste also does not harm the environment. An example of a natural dye is young teak leaves. This type of research is experimental research, the experimental method is a method used to determine the appearance of hematoxylin eosin staining in the kidneys of white rats using teak leaf extract as an alternative to eosin. In the treatment group, the coloring using teak leaf extract was very striking because teak leaves contain anthocyanin so the color is very red. From the results of statistical data, it can be seen that teak leaf extract with a concentration of 1:2 and 1:3 is better than teak leaf extract with a concentration of 1:4. From the results of observations, coloring using teak leaf extract with a concentration of 1:4 has experienced a decrease in the quality of the coloring. The results of teak leaf extract with concentrations of 1:2 and 1:3 can be used as an alternative to add eosin if eosin stock is limited or eosin is difficult to obtain. But eosin is still the gold standard in the coloring process. Teak leaf extract with a concentration of 1:4 cannot be used as an alternative to add eosin because the quality of the staining results is not very clear, the nuclei and cytoplasm are pale.

Keywords: Teak Leaf Extract, Eosin, Hematoxilin Eosin Staining, White Rat Kidney