PERBANDINGAN PENGGUNAAN XILENA DAN MINYAK TANAH PADA PROSES DEPARAFINISASI DAN PENJERNIHAN DALAM PEWARNAAN HEMATOKSOSILIN EOSIN TERHADAP GINJAL TIKUS PUTIH

Hematoxylin and Eosin staining is the most commonly used staining technique in histological tissue staining. The initial stage involves the Deparaffinization process, which aims to remove excess paraffin from tissues or glass slides, allowing clear visualization of the tissues. Xylene is the solution used in the Deparaffinization process, and it is not only employed for deparaffinization but also for cleaning purposes. The cleaning process aims to make the morphological structure of the sample appear clearer, transparent, and more easily observable under a microscope. Xylene solution is considered the gold standard in Hematoxylin and Eosin staining for deparaffinization and clearing processes due to its ability to dissolve paraffin in tissues. Xylene is derived from petroleum, specifically from the aromatic hydrocarbon group. However, its usage has drawbacks, as it is a carcinogenic compound and relatively expensive. As an alternative, experiments were conducted using kerosene as a substitute for xylene in the deparaffinization and clearing processes. Kerosene shares some properties with xylene and is commonly used as a cleaning agent due to its effective fat and dirt dissolution. Kerosene has the advantage of being more cost-effective than xylene. Given its similar properties and lower cost, researchers considered kerosene as a potential alternative to xylene, evaluating the quality of tissue preparations. The research employed a True Experimental method, conducted in two groups: the experimental group using kerosene and the control group using xylene as a comparative measure. Twenty white rat kidneys served as test samples for deparaffinization and clearing processes with both xylene and kerosene. Results from deparaffinization and clearing with xylene demonstrated good histological outcomes. In contrast, the quality of deparaffinization and clearing with kerosene solution yielded unsatisfactory results. The research revealed poorly prepared specimens with remnants of paraffin in tissues, preventing the optimal penetration of the dye and potentially disrupting tissue assessment during microscopic
readings. Consequently, it can be concluded that kerosene cannot serve as a viable alternative to xylene, despite sharing some properties, as it does not produce satisfactory results comparable to xylene.

Keywords: Deparaffinization, Clearing, Xylene, Kerosene