PERBANDINGAN HASIL PEMERIKSAAN GLUKOSA DARAH SEWAKTU PADA SERUM SEGERA DAN DIPERIKSA BERSELANG 40 MENIT SAMPAI MENIT KE-160 METODA GLUCOSEOXIDASE-PARA AMINO PHENAZONE

Pre-analytical stages such as sample preparation are important stages in clinical laboratory examinations. Temporary blood glucose tests can be done at any time or do not require fasting preparation. Blood glucose examination to confirm the diagnosis of diabetes mellitus. Pre-analytical stages such as sample preparation are important stages in clinical laboratory examinations. Serum or plasma that will be used for blood glucose examination must be immediately separated from blood cells because leukocyte and erythrocyte cells will break down glucose for metabolism even though it is outside the body. Serum glucose levels were checked immediately, delayed 40 minutes, delayed 80 minutes, delayed 120 minutes, and delayed 160 minutes. From the results of the examination, it was found that there was a decrease in glucose levels in all serum samples examined. At the 40th minute there was a decrease in glucose levels from the immediate serum glucose value of 7.39%, at 80 minutes there was a decrease of 14.91%, at the 120th minute there was a decrease of 22.56%, and at the 160th minute there was a decrease of 30.40%. These results were then analyzed using the ANOVA test and Bonferroni's follow-up test (significance value at 0.05), it was found that the results of glucose levels from serum immediately until the 80th minute were not significantly different, but starting from the 120th minute onwards, the results of these glucose levels significantly different from the results of immediate serum glucose levels. So for checking glucose while in serum, the recommended time is no more than 120 minutes if the sample is held at room temperature. The room temperature in the laboratory during research was around 23-25oC.

Key words: Temporary glucose, GOD-PAP, serum