PENGARUH PENYIMPANAN SAMPEL DARAH EDTA TERHADAP JUMLAH TROMBOSIT METODE AUTOMATIC DENGAN ALAT HEMATOLOGI ANALYZER

Platelet examination at Puskesmas Rawat Inap Situ was carried out using a Swelab Alfa Basic Series 116326 hematology analyzer with EDTA blood samples. Delay in the EDTA blood test is one of the factors that influences the results of the platelet count test, platelets that are left for >1 hour will stick vey easyly so that when examined using a hematology analyzer it can show a lower count because giant platelets and platelets that stick together are not counted. The aim of this research is to determine the effect of delay time and storage temperature on the results of platelet count examination. This research is a type of quasi-experimental time series quantitative analysis research with random sampling which is then analyzed using two-way ANOVA test to determine the significant influence between the observed data and the significance limit (p<0,05), the subjects of this research were patients from IGD/Ranap units as many as 20 respondents. The p-value obtained was 0,371 (>0,05) so there was no significant influence on the platelet count results based on storage temperature and the p-value was 0,442 (>0,05) so there was no significant influence on the platelet count resulrs based on the delay time. The conclusion of this research is that there is no significant effect on the results of the platelet count examination after being treated with variations in delay time (0, 30 minutes, 60 minutes and 90 minutes) and storage temperature (18-25℃ and 2-8℃).

Keywords : Platelets, Blood with EDTA, Hematology analyzer.