MEMBANDINGKAN JUMLAH LEUKOSIT DENGAN PENGGUNAAN PERASAN JERUK NIPIS (Citrus aurantifolia) 1,5%, 2,5%, 3,5% SEBAGAI PENGGANTI ASAM ASETAT GLASIAL

Turk is a solution used to thin the blood when counting white blood cells (leukocytes). Turk is a solution consisting of a mixture of 2% glacial acetic acid and 1% gentian violet. This study aims to see the number of leukocytes counted with turk's solution as a control and with lime juice (Citrus aurantifolia) with concentrations of 1,5%, 2,5%, 3,5%. This type of research is an experiment with a cross sectional research design carried out in January-February 2024 at the Anatya Medika Clinical Laboratory. The population in this study were all adult female patients with a sample size of 1 person who was repeated five times. Leukocyte examination was carried out using a manual method with a counting chamber (improved Neubauer). The average number of leukocytes using turk solution as a control was 5.216 cells/mm3, while with modified lime juice with a concentration of 1,5% it was 3.295 cells/mm3, lime juice with a concentration of 2,5% was 4.095 cells/mm3, and lime juice with a concentration of 3,5% is 4.254 cells/mm3. From the research results it can be concluded that the use of modified lime juice (citrus aurantifolia) 1,5%, 2,5%, 3,5% is still less effective as a substitute for glacial acetic acid in turk solution. However, in the results of examining leukocytes with lime juice (citrus aurantifolia) at a concentration of 3,5%, the results were closest to turk's solution as a control.

Keywords: Leukocytes, Turk's solution, Lime modification