PERBANDINGAN PEWARNAAN HEMATOXYLIN-EOSIN YANG MENGGUNAKAN NATRIUM BIKARBONAT 0,5% DAN LITHIUM CARBONAT 0,5% PADA PROSES BLUEING

Hematoxylin Eosin staining in the Blueing process uses 0.5% Lithium Carbonate as a control and the Blueing process uses 0.5% Sodium Bicarbonate by conducting experiments on the liver of white mice. The aim of this research is to determine the quality of Hematoxylin Eosin staining using 0.5% Sodium Bicarbonate and 0.5% Lithium Carbonate in the Blueing process. This type of research uses experimental methods. The results of this research are presented in the form of non-parametric tests. To see whether there is a difference, testing is carried out using the Mann�Whitney test. Based on the results of the Mann-Whitney test regarding the difference in Hematoxylin Eosin staining results in the Blueing process between Lithium Carbonate 0.5% and Sodium Bicarbonate 0.5%, the Sig asymp value was obtained. (2-tailed) 0.067, then H0 is accepted and H1 is rejected, which means there is no
significant difference between Haematoxyln Eosin staining in the Blueing process using 0.5% Lithium Carbonate and using 0.5% Sodium Bicarbonate, so 0.5% Sodium Bicarbonate is used as Alternative Blueing agents can be considered because they provide the same quality results as Lithihum Carbonate 0.5%. It is recommended for future researchers to carry out pH measurements to what extent pH influences the results of the preparation.

Keywords : Haematoxylin Eosin Staining, Sodium Bicarbonate 0.5%, Lithium Carbonate 0.5%